Exposed to Leishmania infantum Infection Different Categories of Dogs Naturally Sampling Associated with Nested PCR for Diagnostic Value of Conjunctival Swab

نویسندگان

  • Marina Gramiccia
  • Antognoni
  • Andrea Onofri
  • Daniela Piergili Fioretti
  • Trentina Di Muccio
  • Fabrizia Veronesi
  • Maria Teresa
  • Maria Teresa Antognoni
چکیده

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منابع مشابه

Diagnostic value of conjunctival swab sampling associated with nested PCR for different categories of dogs naturally exposed to Leishmania infantum infection.

The objective of the present study was to evaluate the diagnostic performance of a noninvasive assay, conjunctival swab (CS) nested-PCR (n-PCR), for diagnosing canine leishmaniasis (CanL) in different stages of infection in comparison to the performance of the indirect immunofluorescence antibody test (IFAT), lymph node microscopy, and buffy coat n-PCR. To this end, we performed a cross-section...

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Canine Skin and Conjunctival Swab Samples for the Detection and Quantification of Leishmania infantum DNA in an Endemic Urban Area in Brazil

BACKGROUND We evaluated kDNA PCR/hybridization and quantitative real-time PCR (qPCR) targeting the gene of DNA polymerase of Leishmania infantum for CVL diagnosis and assessment of parasite load in clinical samples obtained invasively and non-invasively. METHODOLOGY/PRINCIPAL FINDINGS Eighty naturally infected dogs from an endemic urban area in Brazil were used. Animals were divided into two ...

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Evaluating the Accuracy of Molecular Diagnostic Testing for Canine Visceral Leishmaniasis Using Latent Class Analysis

Host tissues affected by Leishmania infantum have differing degrees of parasitism. Previously, the use of different biological tissues to detect L. infantum DNA in dogs has provided variable results. The present study was conducted to evaluate the accuracy of molecular diagnostic testing (qPCR) in dogs from an endemic area for canine visceral leishmaniasis (CVL) by determining which tissue type...

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تاریخ انتشار 2012